SOME IMMUNOLOGICAL CHARACTERISTICS IN MUSTARD EXPOSED PATIENTS
Abstract
Introduction. Mutagenesis, carsinogenesis and cytotoxicity effects on body tissues are side effects of sulfur mustard which in turn, causes the bone marrow and lymphoid system dysfunction. Previous investigation in 31 Iranian chemical casualties, indicated the presence of persistant atypical lymphocytes and the decrease of T lymphocyte subsets and surface markers. The present study was performed to evaluate the changes of B lymphocyte surface markers in the above mentioned group and compare the changes with two other control groups.
Methods.The first control group consisted of 30 chemical casaulities with the same clinical conditions and the same history of exposure to mustard but absence of atypical lymphocytes in their blood, and the second control group were consisted of 30 completely normal indivduals. Monoclonal antibodies congugated to fluoroscein isothiocyanate were used to determine B lymphocyte markers.The monoclonal antibodies used in this study were against human immunoglobuline δ and µ heavy chain, λ and κ light chains, anti human HLADR and anti human CD10. The tests were performed on 100 ml of peripheral blood, using flow cytometer model EPICS®XL.
Results. In comparsion with normal controls, k and λ light chains showed a significant decrease (P < 0.001) while HLADR showed a significant increase (P < 0.001) in the two groups of poison gas injury. No significant difference could be seen in µ and δ chain. Two patients of victims group with atypical lymphocytes were CD10 positive.
Discussion. Results of this investigation on B lymphocyte markers, suggests the increasing risk of neoplasuc or Iymphopro fife rative disorder with B cell origin, in chemical casaulties with mustard gas.
Methods.The first control group consisted of 30 chemical casaulities with the same clinical conditions and the same history of exposure to mustard but absence of atypical lymphocytes in their blood, and the second control group were consisted of 30 completely normal indivduals. Monoclonal antibodies congugated to fluoroscein isothiocyanate were used to determine B lymphocyte markers.The monoclonal antibodies used in this study were against human immunoglobuline δ and µ heavy chain, λ and κ light chains, anti human HLADR and anti human CD10. The tests were performed on 100 ml of peripheral blood, using flow cytometer model EPICS®XL.
Results. In comparsion with normal controls, k and λ light chains showed a significant decrease (P < 0.001) while HLADR showed a significant increase (P < 0.001) in the two groups of poison gas injury. No significant difference could be seen in µ and δ chain. Two patients of victims group with atypical lymphocytes were CD10 positive.
Discussion. Results of this investigation on B lymphocyte markers, suggests the increasing risk of neoplasuc or Iymphopro fife rative disorder with B cell origin, in chemical casaulties with mustard gas.
Keywords
Immunoligical Study, Mustard Gas, Sulfur Mustard, B-Lymphocyte, HLA, Human antibody