lysine, proline, epigallocatechin gallate, zinc and alpha-1-antitrypsin on lung carcinogenesis induced by benzo(a) pyrene in mice

Abstract

• Background: This study was aimed to evaluate protective and therapeutic effects of a specific mixture, containing vitamin C, lysine, proline, epigallocatechin gallate and zinc, as well as alpha-1-antitrypsin protein on lung tumorigenesis induced by benzo(a) pyrene (B (a) P) in mice.
• Materials and Methods: Swiss albino mice were divided into two main experiments, experiment (1) the mice were injected with 100 mg/kg b (a) P and lasted for 28 weeks, while experiment (2) the mice were injected with 8 doses each of 50 mg/kg b (a) P and lasted for 16 weeks. Each experiment divided into fi ve groups, group (I) received vehicle, group (II) received the protector mixture, group (III) received the carcinogen, group (IV) received the protector together with the carcinogen (simultaneously) and group (V) received the carcinogen then the protector (consecutively).
• Results: Total sialic acid, thiobarbituric acid reactive substances, vascular epithelial growth factor, hydroxyproline levels, as well as elastase and gelatinase activities showed significant elevation in group (III) in the two experiments comparing to control group (P < 0.001). These biochemical alterations were associated with histopathological changes. Administration of the protector in group IV and group V causes significant decrease in such parameters when compared with group III in the two experiments (P < 0.001).
• Conclusion: Th e present protector mixture has the ability to suppress neoplastic alteration and restore the biochemical and histopathological parameters towards normal on lung carcinogenesis induced by benzo(a) pyrene in mice. Furthermore, the present mixture have more protective rather than therapeutic action.
• Key words: Alpha-1-antitrypsin, benzo(a) pyrene, epigallocatechin gallate, lung tumorigenesis, lysine, proline, protector, vitamin C, zinc