Associations between high density lipoprotein mean particle size and serum paraoxonase-1 activity

Abstract

• BACKGROUND: High density lipoprotein (HDL) particles are heterogeneous in composition, structure, size, and may differ in conferring protection against cardiovascular disease. HDL associated enzyme, paraoxonase-1 (PON1), has an important role in attenuation of atherogenic low density lipoprotein (LDL) oxidation. The aim of this study was to investigate the associations between HDL particle size and PON1 activity in relation to serum HDL cholesterol (HDL-C) levels.
• MATERIALS AND METHODS: One hundred and forty healthy subjects contributed to this study. HDL was separated by sequential ultracentrifugation and its size was estimated by dynamic light scattering. Paraoxonase activity was measured spectrophotometrically using paraoxon as substrate.
• RESULTS: Results of this study showed that PON1 activity had negative correlations with HDL mean particle size (r = −0.22, P < 0.01), HDL₂/HDL₃ ratio, and serum HDL-C levels (r = −0.25, P < 0.01). HDL mean particle size and HDL₂/HDL₃ ratio had negative correlation with body mass index (BMI), waist hip ratio (WHR), and serum triglyceride (TG) levels, and positive correlation with serum HDL-C levels. Serum HDL-C levels had significant positive correlations with age, total cholesterol (TC), and apolipoprotein A-I (apo A-I) and significant negative correlation with BMI, WHR, and TG.
• CONCLUSION: Based on the results of this study, determination of HDL mean particle size beside the serum PON1 activity may help to better understand the CAD risks, pathogenesis, and prognosis, and may also help to design therapeutic protocols toward beneficial modifications of HDL characteristics.
• Key words: Dynamic light scattering, HDL size, HDL-C, PON1 activity, zetasizer