Scolicidal effects of Squash (Corylus spp) seeds, Hazel (Curcurbia spp) nut and Garlic (Allium sativum) extracts on hydatid cyst protoscolices

Abstract

• Background: Because there is no effective drug therapy for hydatid cyst yet, assessment and finding of some new agents especially from herbal origin with a desired scolicidal effect attracts great attention for treatment and pre-surgical use to prevent the hydatid cyst recurrence. Hazelnut, squash seeds and garlic chloroformic and hydro-alcoholic extracts’ scolicidal effects were examined.
• Materials and Methods:  Suspension of protoscolices was obtained from infected liver and or lung of sheep and goats from Ziyaran abattoir. The chloroformic and hydro-alcoholic extracts from hazelnut, squash seeds and garlic were extracted using the succilate method. Scolicidal effect of each extract assessed in different concentrations and effected time using microscopy and 0.1% eosin solution stained only killed protoscolices.
• Results: Present study showed that garlic had more potent scolicidal effects among all the 3 plants and the chloroformic extract of garlic was the most potent protoscolicid among all of the extracts and killed 98% of protoscolices in 50 mg/ml on a minimum of 20 minutes of exposure.
• Conclusion: Garlic chloroformic extract is a safe and potent protoscolicid and might be used in hydatid cyst treatment and pre-surgery to prevent secondary cyst recurrence.
• Keywords: Chloroformic, cystic echinococcosis, extract, garlic, hazelnuts, hydro-alcoholic, protoscolicid effect, squash seed