The effect of omega-3 fatty acids on serum paraoxonase activity, vitamins A, E, and C in type 2 diabetic patients

Anis Kouchak, Mahmoud Djalali, Mohamadreza Eshraghian, Ahmad Saedisomeolia, Abolghassem Djazayery, Hossein Hajianfar


  • BACKGROUND: Diabetes mellitus is a heterogeneous metabolic disorder characterized by hyperglycemia. Studies showed paraoxonase activity, and vitamin C and A levels are decreased in diabetes. The effect of omega-3 fatty acids on serum paraoxonase activity and vitamins A, E, C in patients with type 2 diabetes is not fully understood. This study aimed to determine the effect of omega-3 fatty acids on paraoxonase activity, vitamins C, A and E levels in type 2 diabetic patients.
  • METHODS: In a double-blind, placebo controlled trial, 80 type 2 diabetic patients were randomly enrolled into the study. Study subjects received daily 2714 mg of omega-3 fatty acids or placebo for 8 weeks. Ten milliliter fasting blood was collected before and after treatments. Serum paraoxonase activity and vitamin C levels were measured by spectrophotometry. Vitamin A and vitamin E were measured using high performance liquid chromatography. Nutrient intake was estimated using 24-hours dietary recall questionnaire (for 2 days) before and after treatments. Dietary data were analyzed using FPII. To compare the means of variables between the two groups, independent t-test was employed. Differences between variables before and after interventions were calculated using paired t-test.
  • RESULTS: Serum levels of paraoxonase activity were significantly increased after omega-3 intake (126.47 IU/ml vs. 180.13 IU/ml). However, omega-3 intake caused no significant change in serum vitamin A, C, and E.
  • CONCLUSIONS: Supplementation of omega-3 fatty acids was found to increase paraoxonase activity in diabetic patients.
  • KEYWORDS: Paraoxonase, Diabetes mellitus, Vitamin C, Vitamin A, Vitamin E.


paraoxonase, diabetes mellitus, vitamin C, vitamin A, vitamin E

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