SERUM LEVEL OF LIPIDS, APOLIPOPROTEINS AND VITAMIN D IN CHILDREN AT HIGH RISK OF CORONARY ARTERY DISEASE
Abstract
Introduction. Coronary artery disease (CAD) is one of the most prevalent disease in human population that has high prevalence and mortality in lsfahan (Iran). As positive family history and changes in serum lipids and lipoproteins are risk factors of these diseases, and also studies have showed the relationship between serum vitamin D and CAD, we studied serum level of lipids, lipoproteins and vitamin D in high risk children compared with control group, and the relation between serum vitamin D and other factors.
Methods. This case-control study was done on 44 subjects (25 boys, 19 girls) aged 2-18 years old with positive CAD family history as case group and also 44 persons with negative CAD family history as control group with the same age groups. The subjects were selected by convenience sampling method. Children who consumed antiepilepthic drugs as phenytoin or phenobarbital and those who had positive family histroy for renal stone were excluded for variable vitamin D levels due to drug interaction and genetic susceptibility, respectively. All subjects were invited to Isfahan Cardiovascular Research Center. Using a questionnaire, information on personal characteristics, CVD family history and ... were obtained. A fasting (12-14 hr) blood sample was drawn from each one. Serum APOA1, APO B100 and vitamin Dwere measured by radioimmunoassay and serum lipids by ELAN 2000 autoanalyzer. Statistical analysis was done by SPSS. The level of serum APOA1, APO B100 and vitamin D were compared between two groups by independent t test and the relation of the mentioned apolipoproteins with vitamin D was studied using multiple linear regression.
Results. Serum vitamin D was significantly lower in case group (P < 0.045). Among studied factors, only triglyceride was significantly higher in control group (P < 0.0001) and also no significant relaitonship was observed between serum APO A1, APO B100 and vitamin D. Sex comparision in case group showed those mean levels of total and LOL cholesterol, triglyceride and APO B100 in boys are higher than girls are. Instantly in girls, mean ApoA1 and HDL cholesterol are higher than boys are. In addition, in neither case nor control group"s vitamin D showed significat relationship with total, LDL and HDL cholesterol and triglyceride (P > 0.05).
Discussion. Generally, as optimum level of vitamin D is regulated by sereval environmental, genetic and metabolic factors, it is necessary to clear the real role of antioxidant vitamin Dfor decreasing CAD occurence especially in Iran.
Methods. This case-control study was done on 44 subjects (25 boys, 19 girls) aged 2-18 years old with positive CAD family history as case group and also 44 persons with negative CAD family history as control group with the same age groups. The subjects were selected by convenience sampling method. Children who consumed antiepilepthic drugs as phenytoin or phenobarbital and those who had positive family histroy for renal stone were excluded for variable vitamin D levels due to drug interaction and genetic susceptibility, respectively. All subjects were invited to Isfahan Cardiovascular Research Center. Using a questionnaire, information on personal characteristics, CVD family history and ... were obtained. A fasting (12-14 hr) blood sample was drawn from each one. Serum APOA1, APO B100 and vitamin Dwere measured by radioimmunoassay and serum lipids by ELAN 2000 autoanalyzer. Statistical analysis was done by SPSS. The level of serum APOA1, APO B100 and vitamin D were compared between two groups by independent t test and the relation of the mentioned apolipoproteins with vitamin D was studied using multiple linear regression.
Results. Serum vitamin D was significantly lower in case group (P < 0.045). Among studied factors, only triglyceride was significantly higher in control group (P < 0.0001) and also no significant relaitonship was observed between serum APO A1, APO B100 and vitamin D. Sex comparision in case group showed those mean levels of total and LOL cholesterol, triglyceride and APO B100 in boys are higher than girls are. Instantly in girls, mean ApoA1 and HDL cholesterol are higher than boys are. In addition, in neither case nor control group"s vitamin D showed significat relationship with total, LDL and HDL cholesterol and triglyceride (P > 0.05).
Discussion. Generally, as optimum level of vitamin D is regulated by sereval environmental, genetic and metabolic factors, it is necessary to clear the real role of antioxidant vitamin Dfor decreasing CAD occurence especially in Iran.
Keywords
Children, Cardiovascular Disease, Lipid, Lipoprotein, Vitamin D