The relationship between L?leucine?7?amido ?4?methyl coumarin 1 gene polymorphism and susceptibility to the chronic hepatitis B virus infection in an Iranian population
Abstract
Background: Lamnin has important effects on human immunity system. The current study aimed to assess the role of L?leucine?7?amido?4?methyl coumarin 1 gene polymorphisms on hepatitis B virus (HBV) susceptibility.
Materials and Methods: Te rs20558, rs20563, rs10911193, rs10911251, and rs1413390 polymorphisms were analyzed using polymerase chain reaction (PCR) and PCR?reaction–restriction fragment?length polymorphism and amplifcation?refractory mutation system?PCR using three di?erent groups including chronic HBV?infected patients, HBV patients who were resolved their infection spontaneously and healthy volunteers. Laminin concentrations were also measured in the blood of these individuals. Results: People with rs20558C, rs20563G, and rs10911193T alleles have an increased risk of HBV infection. Moreover, we found that CGTAT haplotype was more frequent in chronically infected people who could a?ect the mechanism of disease. Furthermore, there was a signifcant relationship between laminin concentration and rs20558, rs20563, and rs10911193 genotypes in patients. Conclusion: According to the statistical analysis, rs20558, rs20563, rs10911193 polymorphisms probably, related to the chronic HBV infection. In addition, no association of the rs10911251, rs1413390 single nucleotide polymorphisms with the disease was found.
Materials and Methods: Te rs20558, rs20563, rs10911193, rs10911251, and rs1413390 polymorphisms were analyzed using polymerase chain reaction (PCR) and PCR?reaction–restriction fragment?length polymorphism and amplifcation?refractory mutation system?PCR using three di?erent groups including chronic HBV?infected patients, HBV patients who were resolved their infection spontaneously and healthy volunteers. Laminin concentrations were also measured in the blood of these individuals. Results: People with rs20558C, rs20563G, and rs10911193T alleles have an increased risk of HBV infection. Moreover, we found that CGTAT haplotype was more frequent in chronically infected people who could a?ect the mechanism of disease. Furthermore, there was a signifcant relationship between laminin concentration and rs20558, rs20563, and rs10911193 genotypes in patients. Conclusion: According to the statistical analysis, rs20558, rs20563, rs10911193 polymorphisms probably, related to the chronic HBV infection. In addition, no association of the rs10911251, rs1413390 single nucleotide polymorphisms with the disease was found.
Keywords
Hepatitis B virus, laminin, single nucleotide polymorphism